Anti-dimethyl Histone H3 (Arg2) Antibody

产品说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 Arg2 methylation is a widespread silencing modification that inhibits histone H3 Lys4 trimethylation.

特异性

Broad species reactivity is expected.
This antibody recognizes the N-terminus of Histone H3 dimethylated at Arg2.

免疫原

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 dimethylated at Arg2.

应用

Detect Histone H3 using this rabbit polyclonal antibody, Anti-dimethyl Histone H3 (Arg2) Antibody validated for use in western blotting & Dot Blot.
Western Blotting Analysis: A 1:620 dilution of this antibody detected dimethyl Histone H3 (Arg2) in CARM1 treated dimethyl Histone H3 (Arg2) recombinant protein, but demonstrated a loss of signal in untreated dimethyl Histone H3 (Arg2) recombinant protein. (Note: CARM1 is a methyl transferase that results in the methylation of Histone H3.).

Dot Blot (Specificity) Analysis: A 1:620 dilution from a representative lot detected dimethyl Histone H3 (Arg2) peptides, but not unmethylated Histone H3 (Arg2) or other peptides corresponding to modified histones.

Absurance is a trademark of EMD Millipore, Inc.

质量

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:620 dilution from a representative lot detected dimethyl Histone H3 (Arg2) in 10 µg of HeLa acid extract.

目标描述

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Affinity purified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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