Anti-Human CD103 (Ber-ACT8) Colorwheel^® Dye-Ready mAb

产品说明

一般描述

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

特异性

Clone Ber-ACT8 is a mouse monoclonal antibody that detects Integrin alpha-E (CD103). It targets an epitope within the extracellular domain.

免疫原

HTLV-1 induced human MAPS16 T cells

应用

Quality Control Testing:

Evaluated by Flow Cytometry in 7-day PHA-stimulated human PBMCs.

Flow Cytometry Analysis (FC): Staining of one million 7-day PHA-stimulated Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1065, Anti-Human CD103 (Ber-ACT8) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^® Dye (Sold Separately)

目标描述

Integrin alpha-E (UniProt: P38570; also known as HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, CD103) is encoded by the ITGAE gene (Gene ID: 3682) in human. Integrin alpha-E (CD103) is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 19-1124), a transmembrane domain (aa 1125-1147), and a short cytoplasmic domain (aa 1148-1179). It is expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. CD103 expression has been reported on CD4+ regulatory T cells and about 4% of circulating CD8+ T cells also bear the CD103 molecule. Expression of CD103 on alloreactive cells is shown to be augmented by IL-4, IL-10, or TGF-β and decreased by addition of IL-12. It forms a heterodimer with β-7 subunit to form a receptor for E-cadherin and mediate adhesion of intra-epithelial T cells to epithelial cell monolayers. Clone Ber-ACT8 is shown to react with in vitro activated T cells and a small subset of normal resting T cells, but not with resting B cells or several other human cell lines. Its reactivity is restricted to a few T cells in the peripheral blood, the extrafollicular areas of lymph nodes and tonsils, and splenic red pulp. (Ref.: Uss, E., et al. (2006). J. Immunol., 177(5); 2775-2783; Kruschwitz, M., et al. (1991). J. Clin. Pathol. 44(8); 636-645).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

安全信息