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Anti-Human CD15 (28) ColorWheel® Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1001
规格纯度
for use with ColorWheel® Dyes (Required, sold separately)
参考价格
1596.49 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Human CD15 (28) ColorWheel® Dye-Ready mAb
产品介绍:

产品说明

一般描述

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

特异性

Clone 28 is a mouse monoclonal antibody that detects human CD15.

免疫原

Monocytes separated from other peripheral leukocytes on fibronectin plates.

应用

Quality Control Testing:

Evaluated by Flow Cytometry in human blood granulocytes .

Flow Cytometry Analysis: Staining of one million Whole human blood granulocytes was performed using 5 μl of a 1:1 mixture of Cat. No. CWA-1001, Anti-CD15 Antibody (28) ColorWheel® Mouse mAb and CW-PE ColorWheel® activated Phycoerythrin (PE) Dye or an equivalent amount of Mouse IgM isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel® Dye (Sold Separately)

目标描述

Alpha-(1,3)-fucosyltransferase 4 (UniProt: P22083; also known as ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, CD15) is encoded by the FUT4 (also known as ELFT, FCT3A) gene (Gene ID: 2526) in human. CD15 is a single-pass type II membrane protein of the glycosyltransferase 10 family. It contains a cytoplasmic domain (aa 1-147), a transmembrane domain (aa 148-172), and a lumenal domain (aa 173-530). It consists of a trisaccharide with the structure Gal-β(1-4)-(Fuc- (1-3))-GlcNAc and is related to another structure, sialyl-CD15 (NeuNAc- (2-3)-Gal-β(1-4)-(Fuc- (1-3))-GlcNAc; CD15s or sLex, where s refers to sialylated ), which differs only by the addition of a sialic acid linkage to the galactose in the core Lex trisaccharide. CD15 catalyzes alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. It is shown to be a distinguishing marker for human myeloid cells, and it mediates neutrophil adhesion to dendritic cells. It is also involved in neutrophil functions, such as cell-cell interactions, phagocytosis, stimulation of degranulation, and respiratory burst. It is also considered as an important marker for neoplastic Reed Sternberg cells and can be useful for the diagnosis of Hodgkin s lymphoma. (Ref.: Gadhoum, SZ., and Sackstein, R (2008). Nat. Chem. Biol. 4(12); 751-757; Nakayama, F., et al. (2001).Glycobiol. Extracellular Matrices. 276(19); 16100-16106).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆28, monoclonal
产品线ColorWheel®
形式lyophilized
分子量calculated mol wt 59.08 kDa
species reactivityhuman
包装antibody small pack of 25 μL
technique(s)flow cytometry: suitable
同位素/亚型IgMκ
表位序列Unknown
Protein ID登记号NP_002024.1
UniProt登记号P22083
相容性for use with ColorWheel® Dyes (Required, sold separately)
运输ambient
储存温度2-8℃
Gene Informationhuman ... FUT4(2526)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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