Anti-Mouse CD41 (MWReg30) Colorwheel^® Dye-Ready mAb

产品说明

一般描述

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

特异性

Clone MWReg30 is a rat monoclonal antibody that detects murine Integrin alpha-IIb (CD41). It targets an epitope within the extracellular domain.

免疫原

Mouse platelets.

应用

Quality Control Testing:

Evaluated by Flow Cytometry in platelet-rich mouse plasma.

Flow Cytometry Analysis (FC): Staining of one million platelet-rich mouse plasma was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1046, Anti-Mouse CD41 (MWReg30) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of a PE-conjugated rat IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^® Dye (Sold Separately)

目标描述

Integrin alpha-IIb (UniProt: Q9QUM0; also known as GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41) is encoded by the Itga2b gene (Gene ID: 16399) in murine species. Integrins are heterodimeric integral membrane proteins composed of a variable a subunit and a conserved b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. CD41 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-988), a transmembrane domain (aa 989-1014), and a short cytoplasmic domain (aa 1014-1033). Integrin a-IIb (CD41) heterodimerizes with b3 subunit to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It is reported to recognize the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation this receptor brings about platelet/platelet interaction through binding of soluble fibrinogen that leads to rapid platelet aggregation, which physically plugs ruptured endothelial cell surface. (Ref.: Nieswandt, B., et al. (1999). Blood. 94(2); 684-693).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

安全信息