Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

产品说明

一般描述

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged
Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

特异性

Other species cross-reactivity not tested.
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

免疫原

Epitope: C-terminal
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

应用

Research Category
Epitope Tags & General Use
Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
Research Sub Category
Epitope Tags

质量

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

目标描述

Varies depending upon the protein which is tagged.

外形

Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon^®, 0.2 mg/mL BSA) and 50% glycerol.
Protein G Purified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H317 - H411
预防措施声明	P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
危险分类	Aquatic Chronic 2 - Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	390.2 °F - Information refers to the main component.
闪点(C)	199 ℃ - Information refers to the main component.