产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Broad species cross-reactivity is expected.
Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.
免疫原
Epitope: Ser10
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.
应用
Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.
质量
Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.
目标描述
17 kDa
外形
Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.
Protein G Purified
储存及稳定性
Stable for 1 year at from date of receipt.
分析说明
Control
Mitotic HeLa cells (IF).
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | FITC conjugate |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 3H10, monoclonal |
species reactivity | human |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NP_003484 |
UniProt登记号 | Q16695 |
运输 | wet ice |
Gene Information | human ... HIST1H3F(8968) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich