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位置:首页 > 品牌 > Sigma-Aldrich > Anti-trimethyl Histone H3 (Lys27) Antibody

Anti-trimethyl Histone H3 (Lys27) Antibody

品牌
Sigma-Aldrich
货号
ABE44
规格纯度
from rabbit, purified by affinity chromatography
参考价格
7365.52 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-trimethyl Histone H3 (Lys27) Antibody
H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3
产品介绍:

产品说明

一般描述

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.

特异性

Broad species cross-reactivity expected.
This antibody recognizes Histone H3 methylated at Lys27.

免疫原

Branch peptide corresponding to human Histone H3 methylated at Lys27.
Epitope: Lys27

应用

Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.

质量

Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.

Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .

目标描述

~ 17 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract and recombinant Histone H3

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
technique(s)dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登记号NP_003484.1
UniProt登记号Q16695
运输wet ice
Gene Informationhuman ... HIST1H3F(8968)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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