产品说明
一般描述
Affinity purified rabbit anti-sheep IgG, Peroxidase conjugated
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
应用
EIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
This Rabbit anti-Sheep IgG Antibody, HRP conjugate is validated for use in ELISA, WB for the detection of Sheep IgG.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
外形
RECONSTITUTION:
Reconstitute with 1.5 mL of sterile distilled water.
ImmunoAffinity Purified
储存及稳定性
STORAGE: Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.46 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | peroxidase conjugate |
抗体形式 | affinity purified immunoglobulin |
antibody product type | secondary antibodies |
克隆 | polyclonal |
species reactivity | sheep |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
运输 | wet ice |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich