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Raf-1 RBD Protein, GST, 300 μg

品牌
Sigma-Aldrich
货号
14-863
规格纯度
GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity 50% at full length molecular weight 42 kDa. For use in Affinity Binding Assays
参考价格
7760.51 *本价格含增值税费
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数量
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产品名称:
Raf-1 RBD Protein, GST, 300 μg
产品介绍:

产品说明

一般描述

Product Source: Expressed in E. coli
GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity >50% at full length molecular weight 42 kDa.
Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.

应用

Specifically binds to and precipitates Ras-GTP from cell lysate.Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
Research Sub Category
Obesity

Metabolic Disorders
Research Category
Metabolism

质量

Affinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 μg of Raf-1-RBD, GST per well to capture 25-100 μg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424).
Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.

外形

Glutathione-agarose
300 μg of Raf-1 RBD, GST in 150 μL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol.

储存及稳定性

1 year at -20°C from date of shipment.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160405
NACRESNA.41

产品性质

质量水平100
生物来源human
测定>50%
分子量Mw 42 kDa
manufacturer/tradenameUpstate®
technique(s)affinity binding assay: suitable
NCBI登记号NM_002880.2
UniProt登记号P04049
运输dry ice
Gene Informationhuman ... RAF1(5894)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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