产品说明
一般描述
Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.
应用
Coat tissue culture ware with Rat Tail Collagen Solution to culture surface ratio of 5 ug/cm2. For example: 125 ug (2.5 ml) for a T-25 flask. 10.5 ug (210 ul) for each well of a 24-well plate.
Coat tissue culture ware at 37oC for 2 hours. Carefully aspirate Collagen Coating Solution. Rinse the coated surface twice with PBS. Coated tissue culture ware may be used immediately or air-dried and stored at 4 oC for up to one week.
基本信息
NACRES | NA.75 |
产品性质
质量水平 | 100 |
无菌性 | sterile; sterile-filtered |
形式 | liquid |
保质期 | 6 mo. |
包装 | pkg of 20 mL |
technique(s) | cell culture | mammalian: suitable |
UniProt登记号 | P02454 |
运输 | wet ice |
储存温度 | 2-8℃ |
Gene Information | rat ... Col1a1(29393) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich