产品说明
一般描述
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
应用
PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
包装
Kit capacity: 30 assays
组分
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
质量
Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32161000 |
产品性质
质量水平 | 100 |
manufacturer/tradename | Upstate® |
technique(s) | activity assay: suitable affinity binding assay: suitable |
NCBI登记号 | NM_001664.2 |
UniProt登记号 | P08134 P61586 P62745 |
detection method | chemiluminescent colorimetric fluorometric |
运输 | dry ice |
Gene Information | human ... RHOA(387) |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H318 - H410 |
预防措施声明 | P273 - P280 - P305 + P351 + P338 - P391 - P501 |
危险分类 | Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
Sigma-Aldrich