Anti-Bax (NT) Antibody

产品说明

一般描述

BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

特异性

Rat (90%), Mouse (86%).
This antibody recognizes BAX.

免疫原

KLH-conjugated linear peptide corresponding to human BAX at and around the N-terminus.

应用

Anti-Bax Antibody is an antibody against Bax (N-terminus) for use in WB, IP, IC, IH.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated BAX from 500 µg of HL60 cell lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.

质量

Evaluated by Western Blot in HL60 cell lysate.

Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.

目标描述

~ 21 kDa

联系

Replaces: 06-499

外形

Format: Purified
Protein A purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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