产品说明
特异性
Reacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
免疫原
Affinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: immediate early
应用
Immunohistochemistry.
Immunocytochemistry.
Western Blot 1:100
IFA at 1:600-1:1,200 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Final working dilutions must be determined by end user.
Research Sub Category
Infectious Diseases - Viral
Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P).
Research Category
Infectious Diseases
联系
Replaces: MAB8130
外形
Purified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered.
Format: Purified
储存及稳定性
Maintain at +4°C for up to 12 months
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 300 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 6F8.2, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG2a |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich