产品说明
特异性
The antibody reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin (Pierschbacker et al., 1981) comprimising the cell attachment site (Pierschbacker et al., 1981; Pierschabacker et al., 1983, 1984; Hayman et al., 1985; Ruoslahti et al., 1986; Hynes, 1985). It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface (Pierschbacker et al., 1981). Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser (Pierschabacker et al., 1984; Hayman et al., 1985; Ruoslahti et al., 1986).
免疫原
Epitope: cell attachment fragment
Purified human-fibronectin from amnion liquid.
应用
Immunocytochemistry: (5-20 μg/mL) Immunohistochemistry: (5-20 μg/mL) (Not tested on paraffin sections) Immunoblotting: (5-20 μg-mL)
ELISA
Inactivation or inhibition studies in cell culture.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3 is an antibody against Fibronectin Frag for use in ELISA, WB, IC, IH.
Research Sub Category
ECM Proteins
外形
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Format: Purified
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 3E3, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1 |
UniProt登记号 | P02751 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich