Anti-phospho Cyclin-dependent kinase 7 (Thr170) Antibody

产品说明

一般描述

Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin protein and they mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Cyclin-dependent kinase 7 (CDK7), also known as Cyclin-dependent kinase 7, or 39 kDa protein kinase (p39-Mo15), CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, or TFIIH basal transcription factor complex kinase subunit, and encoded by the gene CDK7/CAK/CAK1/CDKN7/MO15/STK1, is a serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CDK7 is inactivated by phosphorylation, thus Cyclin-dependent kinase 7 (Thr107) is an inactive CDK7 protein.

免疫原

Linear peptide corresponding to human phosphorylated phospho Cyclin-dependent kinase 7 (Thr170).
Epitope: Phosphorylated T107

应用

Research Sub Category
Nuclear Receptors
This Anti-phospho Cyclin-dependent kinase 7 (Thr170) antibody is validated for use in western blotting & ChIP for the detection of phospho Cyclin-dependent kinase 7 (Thr170).
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected phospho Cyclin-dependent kinase 7 (Thr170) in Cdk7 and Csk1 kinase (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho Cyclin-dependent kinase 7 (Thr170) in HCT116 cell lysate treated with doxorubicin (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho Cyclin-dependent kinase 7 (Thr170) in 10 µg of A431 cell lysate.

目标描述

~ 39 kDa observed

外形

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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