您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

品牌
Sigma-Aldrich
货号
MABE229
规格纯度
clone CP3.2, from mouse
参考价格
5016.79 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2
Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
产品介绍:

产品说明

一般描述

Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

特异性

This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

免疫原

Epitope: Phosphorylated Thr14 and phosphorylated Tyr15
Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function
Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

质量

Evaluated by Western Blot in UV treated and untreated HeLa cell lysates.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.

目标描述

~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.

外形

Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
UV treated and untreated HeLa cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆CP3.2, monoclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001777
UniProt登记号P06493
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品