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位置:首页 > 品牌 > Sigma-Aldrich > Anti-PPP1CA Antibody, clone 5E9

Anti-PPP1CA Antibody, clone 5E9

品牌
Sigma-Aldrich
货号
MABN690
规格纯度
ascites fluid, clone 5E9, from mouse
参考价格
5180.56 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-PPP1CA Antibody, clone 5E9
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
产品介绍:

产品说明

一般描述

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1A) encoded by the gene named PPP1CA or PPP1A is one of the three catalytic subunits of protein phosphatase 1 (PP1), one of the major protein phosphatases in the cell. PPP1CA interacts with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate a huge variety of target proteins. PPP1CA is critical for cell division, chromatin structure, protein synthesis, glycogen metabolism, muscle and cardiac function as well as the regulation of long-term ionic conductances and even synaptic plasticity. Developmentally it has nearly as many roles with principal ones being as a regulator of neural tube and optic fissure closure as well as enteric neural crest cell (ENCCs) migration during development. PPP1CA can be found both cytoplasmically and nuclear localized depending upon its regulatory partner but PPP1CA is mostly nuclear/nucleoplasm localized. Interestingly in the brain, PPP1CA also functions as a suppressor of learning and memory and as a potential mediator of cognitive decline during aging. EMD-Millipore’s Anti-PPP1CA mouse monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HeLa, HepG2, MCF-7, Jurkat and A549 cells and by direct ELISA against varying concentrations of antigen.

免疫原

Purified recombinant fragment of human PPP1CA expressed in E. Coli.

应用

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-PPP1CA Antibody, clone 5E9 is a highly specific mouse monoclonal antibody & that targets Serine/threonine-protein phosphatase & has been tested in western blotting.

质量

Evaluated by Western Blotting in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPP1CA in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

Optimal working dilutions must be determined by end user.

目标描述

~38 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆5E9, monoclonal
species reactivityhuman
technique(s)western blot: suitable
同位素/亚型IgG1
UniProt登记号P62136
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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