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Anti-TRAF1 Antibody, clone 1F3

品牌
Sigma-Aldrich
货号
MABC260
规格纯度
clone 1F3, from rat
参考价格
6190.47 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-TRAF1 Antibody, clone 1F3
TNF receptor-associated factor 1, TNF receptor-associated factor 1
产品介绍:

产品说明

一般描述

TNF receptor-associated factor 1 (UniProt: Q13077; also known as Epstein-Barr virus-induced protein 6, TRAF1) is encoded by the TRAF1 (also known as EB16) gene (Gene ID: 7185) in human. TRAF1 is homodimeric pro-survival adapter molecule that regulates the activation of NF-kB and JNK. It can also exist as a heterotrimer with TRAF2. It plays a role in the regulation of cell survival and apoptosis. The heterotrimer form is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. The C-terminal region of TRAF1 contains the TRAF domain (aa 266-412) that binds to receptor cytoplasmic domains. It also has a coiled coil domain (aa 182-264) that mediates its homo- and heterotrimerization. TRAF1 is also reported to essential for memory T cell survival. TRAF1 protein level is significantly reduced in HIV-specific CD8 T cells with progression of infection. Its levels can increase following treatment with IL-7. Hence, TRAF1 levels could serve as a useful parameter in monitoring effectiveness of anti-HIV therapies. (Ref.: Wang, C., et al. (2018). J. Immunol. 200(2); 558-564).

免疫原

GST-tagged recombinant protein corresponding to human TRAF1.

应用

Research Sub Category
Apoptosis - Additional
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in certain cell lines (Siegler, G., et al. (2003). J Clin Pathol: Mol Pathol. 56:156-161.).
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in PBMCs and in certain T cells from HIV-infected individuals (Wang, C., et al. (2011). JEM.1-15.).
Anti-TRAF1, clone 1F3 detects levels of TRAF1 proteins & has been published & validated for use in WB, IHC & Flow.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blot in B95-8 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected TRAF1 in 10 µg of B95-8cell lysate.

目标描述

~46 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~33 kDa

外形

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
B95-8cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆1F3, monoclonal
species reactivitymonkey, human
technique(s)flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001177874
UniProt登记号Q13077
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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