产品说明
一般描述
MANF has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease.
生化/生理作用
MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues.
外形
Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | 15-25 μg/mL ED50 |
| 分子量 | 18.1 kDa |
| 包装 | pkg of 25 μg |
| 杂质 | <0.1 EU/μg endotoxin, tested |
| 颜色 | white to off-white |
| UniProt登记号 | P55145 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MANF(7873) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
