产品说明
一般描述
Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
生化/生理作用
The enzymatic activity of macrophage migration inhibitory factor (MIF) is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.
外形
Lyophilized from 10mM Sodium Phosphate, pH 7.0.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
基本信息
NACRES | NA.32 |
产品性质
生物来源 | human |
重组 | expressed in insect cells |
测定 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
形式 | lyophilized |
分子量 | 15.0 kDa |
包装 | pkg of 25 μg |
杂质 | endotoxin, tested |
UniProt登记号 | P14174 |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... MIF(4282) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich