产品说明
一般描述
The gene SFRP1 (secreted frizzled related protein 1) is mapped to human chromosome 8p22–p12, a region frequently deleted in breast cancer. It is highly expressed in normal breast tissue.
生化/生理作用
Secreted Frizzled-related protein 1 (SFRP1) is a secreted inhibitor of Wnt signaling pathway. DNA damage or oxidative stress causes cellular senescence, which leads to overexpression of sfrp1. This protein induces senescence phenotypes via the inactivation of Wnt signaling and activation of the retinoblastoma (Rb) pathway. Mutations or aberrant methylation in this gene impair the induction of senescence in several cancers, including breast cancer, bladder cancer, and colorectal tumorigenesis.
序列
SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK
外形
Lyophilized from 10 mM Sodium Phosphate pH 7.0.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
产品性质
| 生物来源 | human |
| 重组 | expressed in HeLa cells |
| 测定 | ≥97% (HPLC) ≥97% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | 0.3-0.5 μg/mL ED50 |
| 分子量 | 31.0-36.0 kDa |
| 包装 | pkg of 20 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | <0.1 EU/μg endotoxin, tested |
| 颜色 | white |
| UniProt登记号 | Q8N474 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SFRP1(6422) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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