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SLIT-2-N human

品牌
Sigma-Aldrich
货号
SRP3155
规格纯度
recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
参考价格
4752.23 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
SLIT-2-N human
SLIL3
Slit homolog 2 protein
产品介绍:

产品说明

一般描述

Slit guidance ligand 2 (SLIT-2) is a member of the Slit family that signals through the Roundabout (Robo) receptor. It is also referred to as SLIL3. SLIT2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. It is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1093 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0kDa by SDS-PAGE analysis under reducing conditions.

应用

Slit guidance ligand 2 (SLIT-2) has been used to treat cells in podocyte culture and in myosin II regulatory light chain (MRLC) phosphorylation assay.

生化/生理作用

Slit guidance ligand 2 (SLIT-2) acts as a repellent for axon guidance and neuronal migration, and it can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. It prevents the migration of T cells, neutrophils and macrophages, but enhances eosinophil migration. The protein has been shown to be downregulated in cancers.

序列

QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNLPETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAFQ GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRVDAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSAKEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKITDIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS NRITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLLANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AIQDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIPRD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFSNMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGAF NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGPGEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEEDGFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGEHCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP PMV

外形

Lyophilized from 20 mM Tris + 200 mM L-Arginine, pH 8.8.

重悬

Centrifuge the vial prior to opening. Reconstitute in 20mM Tris, pH 8.8 + 150mM NaCl to a concentration of 0.1-1.0mg/ml. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in HEK 293 cells
测定≥98% (HPLC)
≥98% (SDS-PAGE)
形式lyophilized
分子量 120.0-140.0 kDa
包装pkg of 200 μg
pkg of 25 μg
technique(s)cell culture | mammalian: suitable
杂质<0.1 EU/μg endotoxin, tested
颜色 white
UniProt登记号O94813
运输wet ice
储存温度−20℃
Gene Informationhuman ... SLIT2(9353)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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