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Anti- Neurofilament 200-FITC antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200811
规格纯度
clone NE14, purified from hybridoma cell culture
参考价格
1723.67 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti- Neurofilament 200-FITC antibody, Mouse monoclonal
H-subunit
NF-H
产品介绍:

产品说明

一般描述

Neurofilaments are type of intermediate filaments (IFs) that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. IFs are components of most eukaryotic cells and significantly differ from other cytoskeletal elements of the cell, namely microtubules and microfilaments.

特异性

Monoclonal Anti-Neurofilament 200, also known as Neurofilament-H or Heavy subunit, specifically recognizes the phosphorylated H tail of Neurofilament 200 and shows no reactivity on enzymatically dephosphorylated neurofilaments.2 The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human1, pig1, mouse3, rat4, chicken3, guinea pig3, feline3 and bovine3 origin.

免疫原

Neurofilaments purified from pig spinal cord

应用

The antibody may be used in various immunochemical techniques including Immunohistochemistry and Immunoblotting (~200 kDa).1-7

生化/生理作用

Neurofilaments undergo post-translational modifications including different levels of phosphorylation, which has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit), NF-H, NEFH or 200 kDa neurofilament protein, has an important function in mature axons that is not subserved by the two smaller neurofilament proteins.

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

免责声明

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源mouse
偶联物FITC conjugate
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆NE14, monoclonal
形式buffered aqueous solution
分子量 200 kDa
species reactivityguinea pig, rat, bovine, mouse, feline, chicken, human, pig
包装antibody small pack of 25 μL
浓度~1 mg/mL
technique(s)immunohistochemistry: 1:200-1:400 (4-8 ug/mL) using enzyme treated formalin-fixed, paraffin-embedded rat Cerebellum sections
运输dry ice
储存温度−20℃
Gene Informationhuman ... NEFH(4744)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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