Anti-5-methylcytosine Antibody, clone 33D3

产品说明

一般描述

5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.

特异性

Predicted to react with multiple species.
This antibody detects 5-Methylcytosine, but not unmethylated cytosine.

免疫原

Ovalbumin-conjugated 5-methylcytosine

应用

Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.
IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53

IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693

Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262

MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

质量

Evaluated by Dot Blot in methylated and unmethylated BRCA1.

Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.

目标描述

Detects methylated cytosine

联系

Replaces: MABE527

外形

Protein A
Format: Purified
Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Methylated and unmethylated BRCA1

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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