产品说明
一般描述
Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
免疫原
hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes
应用
Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.
生化/生理作用
Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
储存及稳定性
Store at -20℃. For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
其他说明
In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00145889 |
| NACRES | NA.41 |
产品性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | MM2/193, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~42 kDa |
| species reactivity | monkey, canine, rat, human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | western blot: 1-2 μg/mL using HeLa cell extracts |
| 同位素/亚型 | IgM |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
