产品说明
一般描述
Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.
免疫原
SDS-extracted protein fraction of human myocardium.
应用
The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.
生化/生理作用
Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | HHF-35, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~42 kDa |
| species reactivity | monkey, bovine, mouse, rat, canine, chicken, hamster, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunocytochemistry: 2-4 μg/mL using A10 cells immunohistochemistry: suitable western blot: 0.1-0.2 μg/mL using A10 cell extracts |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
