产品说明
一般描述
Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
特异性
Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
免疫原
FFGVGGEEDC-KLH peptide
Epitope: N-terminus FFGVG neoepitopes
应用
Research Category
Cell Structure
Western blot
ELISA
Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins
Inflammation & Autoimmune Mechanisms
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
外形
Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Format: Purified
Protein A purified
储存及稳定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Cartilage, neural tube, and brain tissue
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | AF-28, monoclonal |
species reactivity | bovine, human, rat, mouse, pig |
should not react with | guinea pig, horse |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_001135.2 NM_013227.2 |
UniProt登记号 | P16112 |
运输 | wet ice |
Gene Information | human ... ACAN(176) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich