Anti-Aiolos Antibody, clone 9D10

产品说明

一般描述

Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.

免疫原

Recombinant protein corresponding to human Aiolos.

应用

Research Sub Category
Transcription Factors
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.

目标描述

~ 58-70 kDa observed

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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