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Anti-Ambra1 Antibody

品牌
Sigma-Aldrich
货号
ABC131
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5666.2 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Ambra1 Antibody
Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1
产品介绍:

产品说明

一般描述

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) is a developmental protein that is involved in autophagy, differentiation and nuerogenesis. AMBRA1 regulates autophagy and development of the nervous system, normal cell survival and proliferation, and the control of protein turnover during neuronal development.

特异性

This antibody recognizes the C-terminus of Ambra1. This antibody should recognize isoforms 1 through 5 based upon immunogen design.

免疫原

Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of mouse Ambra1.

应用

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 0.5 µg/mL of a representative lot detected Ambra1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of a representative lot detected Ambra1 in human cell lysates (HeLa & U87MG) and in mouse tissue homogenates (liver, kidney, and embryo), as well as exogenously expressed myc-Ambra1 in lysates from transfected HEK293 cells. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: Target specificity of a representative lot was confirmed by Western blotting analysis of Ambra1-knock mouse embryos and HeLa cells treated with Ambra1 siRNA. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: A representative lot detected decreased Ambra1 expression in tissue homogenates (kidney, liver, and lung) from Ambra1+/- mice when compared with tissue samples from wild-type mice. (Cianfanelli, V., et al. (2015). Nat. Cell Biol. 17(1):20-30).
Anti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.

质量

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ambra1 in 10 µg of MCF7 cell lysate.

目标描述

~140 kDa observed. Uniprot describes 7 isoforms at 143 kDa (isoform 1), 133 kDa (isoform 2), 87 kDa (isoform 3), 84 kDa (isoform 4), 100 kDa (isoform 5), 136 kDa (isoform 6), and 130kDa (Isoform 7). Uncharacterized band(s) may be observed in some cell lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
MCF7 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
species reactivity (predicted by homology)rat (based on 100% sequence homology)
technique(s)western blot: suitable
NCBI登记号NP_7662587
UniProt登记号A2AH22
运输wet ice
Gene Informationhuman ... AMBRA1(55626)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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