Anti-Ankyrin-G Antibody, clone N106/36

产品说明

一般描述

Ankyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain.

特异性

Other homologies: Human (92% sequence homology), and Mouse (87% sequence homology).

免疫原

Recombinant protein corresponding to human Ankyrin-G.

应用

Anti-Ankyrin-G Antibody, clone N106/36 is a highly specific mouse monoclonal antibody & that targets Ankyrin & has been tested in IHC.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Ankyrin-G in rat cerebral cortex tissue.

Immunohistochemistry Analysis: A representative lot detected Ankyrin-G in rat optic nerve tissue.

Immunofluorescence Analysis: A representative lot detected Ankyrin-G in adult rat cortex tissue.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Ankyrin-G in a rat model of TLE mEC layer II neurons (Hargus, N. J., et al. (2011). Neurobiol Dis. 41(2):361-376.)

质量

Evaluated by Immunohistochemistry in rat hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Ankyrin-G in rat hippocampus tissue.

目标描述

480 kDa calculated

外形

Format: Purified
Purified mouse monoclonal IgG2a κ in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat hippocampus tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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