产品说明
一般描述
Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.
特异性
Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).
免疫原
Epitope: a.a. 66-81 of APP {N-terminus}
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.
应用
Immunofluorescence
Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).
Western blot: reducing conditions, 10μg/ml.
Optimal working dilutions and protocols must be determined by the end user.
Research Category
Neuroscience
Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations.
Research Sub Category
Neurodegenerative Diseases
目标描述
110/120/130 kDa
外形
Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
储存及稳定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Brain tissue, brain cell lysates
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 22C11, monoclonal |
species reactivity | human, fish, canine, monkey, pig, mouse, rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_000484.2 NM_201413.1 NM_201414.1 |
UniProt登记号 | P05067 |
运输 | dry ice |
Gene Information | human ... APP(351) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich