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Anti-Astrin/SPAG5, clone 8B2 Antibody

品牌
Sigma-Aldrich
货号
MABC593
规格纯度
clone 8B2, from rat
参考价格
5854.74 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Astrin/SPAG5, clone 8B2 Antibody
Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126
产品介绍:

产品说明

一般描述

Astrin/SPAG5 is a microtubule binding and cytoskeleton associated signaling protein that plays not only a critical role in mitotic spindle and chromosome segregation but also as an important signal transducer in mTOR and GSK3 beta signaling pathways. Astrin/SPAG5 is localized in the cytoplasm and/or mitotic apparatus (in dividing cells) and centrosomes. In non-dividing cells Astrin/SPAG5 is associated with microtubules unless under cellular stress, then it is localized in stress granules. Astrin/SPAG5 is expressed in many tissues but is very high in testis and sperm and is regulated via the cell cycle highest levels are seen in prophase. Recent research has focused on Astrin/SPAG5 critical role in regulating mTORC1 and its effects on growth and survival. When cells are stress Astrin/SPAG5 inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules (SGs), thereby preventing mTORC1-hyperactivation-induced apoptosis. Because cancerous tumor cells are often stressed, this mechanism is believed to be key to their survival, thus Astrin/SPAG5 activity repression is being examined as potential anti-cancer therapy.

免疫原

Linear peptide corresponding to human Astrin/SPAG5.

应用

Research Sub Category
Apoptosis - Additional
Immunocytochemistry Analysis: A representative lot detected Astrin/SPAG5 HeLa cells (Thedieck, K., et al. (2013). Cell. 154:859-874).
Western Blotting Analysis: A representative lot detected Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
This Anti-Astrin/SPAG5, clone 8B2 Antibody is validated for use in Immunocytochemistry and Western Blotting and Immunoprecipitation for the detection of Astrin/SPAG5.
Research Category
Apoptosis & Cancer

质量

Evaluated by Immunocytochemistry in HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Astrin/SPAG5 in HeLa and A431 cells.

目标描述

134 kDa calculated

外形

Format: Purified
Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源rat
抗体形式purified antibody
antibody product typeprimary antibodies
克隆8B2, monoclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2cκ
NCBI登记号NP_006452
UniProt登记号Q96R06
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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