产品说明
一般描述
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
特异性
MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium.
免疫原
Human cytokeratins.
应用
Anti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
Western blot: 1:100-1:1,000
Immunohistochemistry on frozen tissue sections: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200
Optimal working dilutions must be determined by the end user.
外形
Purified immunoglobulin. Purified immunoglobulin in buffer with 0.1% sodium azide.
Format: Purified
储存及稳定性
Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | monoclonal |
species reactivity | rabbit, canine, quail, chicken, rat, human, guinea pig, pig, hamster |
manufacturer/tradename | Chemicon® |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_000424.3 |
UniProt登记号 | P13647 |
运输 | dry ice |
Gene Information | human ... KRT5(3852) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich