Anti-BPTF Antibody, clone 15B1.1

产品说明

一般描述

Nucleosome-remodeling factor (NURF) subunit BPTF (UniProt E9Q6A7 & A2A654; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alzheimer antigen) is encoded by the Bptf (also known as 9430093H17Rik, Falz) gene (Gene ID 207165) in murine species. ATP-dependent chromatin remodeling complexes are classified into four major subfamilies (SWI/SNF, ISWI, CHD, and INO80). NURF is the founding member of ISWI remodeling complexes. Mammalian NURF is composed of BPTF, the ATPase SNF2L, and the Trp-Asp (WD) repeat protein pRBAP46/48, with BPTF being the largest subunit among the three. BPTF PHD finger-mediated binding of H3K4me3 stabilizes the NURF complex on chromatin, resulting in NURF-catalyzed nucleosome sliding. The NURF remodeling complex is essential for mammalian development and executes epigenetic regulation in multiple signaling pathways, including heat shock, TGF-beta/SMAD, JAK/STAT, WNT/beta-catenin, RB, and nuclear hormone receptors.

免疫原

GST-tagged recombinant protein corresponding to mouse BPTF.

应用

Research Sub Category
Nuclear Receptors
This Anti-BPTF Antibody, clone 15B1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BPTF .
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected BPTF in human testis, rat colon, and mouse colon tissue.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BPTF in 10 µg of HeLa cell lysate.

目标描述

~322 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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