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Anti-Cas9 Antibody, clone 7A9

品牌
Sigma-Aldrich
货号
MAC133
规格纯度
clone 7A9, from mouse
参考价格
5520.38 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Cas9 Antibody, clone 7A9
dCas9
产品介绍:

产品说明

一般描述

Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3′-5′ exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.

特异性

MAC133 recognises both Cas9 and dCas9 (nuclease deficient Cas9)

免疫原

N-Terminal domain of Cas9.

应用

Research Category
Secondary & Control Antibodies
Anti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

质量

Evaluated by Western Blotting in HEK293 expressing Flag-Cas9 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.

目标描述

~160 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆7A9, monoclonal
species reactivity (predicted by homology)all
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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