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Anti-CD1d Antibody, clone D5

品牌
Sigma-Aldrich
货号
MABC983
规格纯度
clone D5, from mouse
参考价格
5688.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-CD1d Antibody, clone D5
Antigen-presenting glycoprotein CD1d, R3G1, CD1d
产品介绍:

产品说明

一般描述

Antigen-presenting glycoprotein CD1d (UniProt P15813; also known as CD1d, R3G1) is encoded by the CD1D gene (Gene ID 912) in human. CD1d is an MHC-like glycoprotein expressed on epithelial cells and can signal innate immune responses by presenting lipid antigen to natural killer (NK) and NK T (NKT) cells. Similar to MHC class I heavy chain (HC), CD1d HC is glycosylated and assembled with β2m in ER. Unlike MHC class I HC, misfoled CD1d and CD1d not properly assembled with β2m are not destroyed by ER quality control mechanisms. CD1d plays a role in both innate and adaptive immunity to various bacteria, viruses, fungi, and parasites. Activation of CD1d-restricted invariant NKT cells enhances host resistance to these microbes via direct killing of CD1d-expressing (infected) cells or by promoting interferon gamma release from conventional NK cells and by modulating adaptive immune cells via alteration of Th1/Th2 polarization. Chlamydia trachomatis is shown to prevent CD1d-mediated innate immune destruction of the infected urethral epithelial cells by targeting CD1d for proteolytic degradation. Specifically, the chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d HC, and CPAF-associated CD1d HC is then ubiquitinated for eventual degradation.

免疫原

Epitope: CD1d α1 domain
Pooled CD1d-GST fusion proteins containing α1 domain alone, α1-α2 domains, or α1–α3 domains.

应用

Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: A representative lot detected time-dependent CD1d degradation in CD1d immunoprecipitate from human penile urethral epithelial (PURL) cells following C. trachomatis infection (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Western Blotting Analysis: A representative lot detected exogenously expressed CD1d in CD1d-transfected C1R human lymphoblastoid cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
Immunoprecipitation Analysis: A representative lot immunoprecipitated intact as well as degraded CD1d from human penile urethral epithelial (PURL) cells following C. trachomatis (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed CD1d independent of glycosylation states from lysates of CD1d-transfected FO-1 human melanoma cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
This Anti-CD1d Antibody, clone D5 is validated for use in Western Blotting, Immunoprecipitation for the detection of CD1d.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected CD1d in 10 µg of THP-1 cell lysate.
Note: Longer exposure time is needed when using total lysates for Western blotting applications. Membrane preparation or immunoprecipitation can be employed to enrich membrane target proteins prior to Western blotting.

目标描述

~37/43 kDa observed

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆D5, monoclonal
species reactivityhuman
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号NP_001757
UniProt登记号P15813
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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