Anti-Chymase Antibody, clone B7, biotin conjugated

产品说明

一般描述

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

特异性

Reacts with human mast cell chymase.

免疫原

Chymase purified from human skin.

应用

Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology

外形

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

分析说明

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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