产品说明
一般描述
Cytoskeleton-associated protein 5 (CKAP5) is a member of the TOG/XMAP215 family containing heat repeats and plays a role in kinetochore mircotubule protection from depolymerization by MCAK. CKAP5 is the homolog of the Xenopus protein, XMAP25 (also known as chTog), which plays an essential role in centrosomal assembly. CKAP5 is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Studies show CKAP5 interacts with TACC1, TACC3, and tubulin leading to the hypothesis that it functions in spindle microtuble organization. Hepatomas and colonic tumors have shown overexpression of CKAP5.
免疫原
KLH-conjugated linear peptide corresponding to human CKAP5.
应用
Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:250 dilution from a previous lot detected CKAP5 in RPE1 cells.
Research Sub Category
Cytoskeleton
Anti-CKAP5 Antibody is an antibody against CKAP5 for use in IC.
质量
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CKAP5 in A431 cells.
目标描述
226 kDa calculated.
Three isoforms are produced by alternative splicing with molecular weights of 225, 218, and 226 kDa
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
A431 cells
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable |
NCBI登记号 | NP_001008938 |
UniProt登记号 | Q14008 |
运输 | wet ice |
Gene Information | human ... CKAP5(9793) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich