产品说明
特异性
The antibody reacts with both native and denatured human collagen type II.
Cross Reactivity Percent
Human collagen type II 90%
Human collagen type I, III, IV, V <1%
Human plasma proteins do not interfere with binding to collagen.
免疫原
Collagen type II extracted and purified from human cartilage.
应用
Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.
Research Category
Cell Structure
ELISA 1:1000.
Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20
Western Blot 1:100
Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins
外形
Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.
Product is liquid in 0.15M sodium chloride, 10mM sodium phosphate pH 7.5 with 0.1% mannitol and 0.1% dextran as stabilizers. No preservative.
储存及稳定性
Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NM_001844.4 NM_033150.2 |
UniProt登记号 | P02458 |
运输 | wet ice |
Gene Information | human ... COL2A1(1280) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich