产品说明
特异性
The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
免疫原
Human placental collagen type III
应用
ELISA 1:3,000
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III.
外形
500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Format: Purified
储存及稳定性
Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | fractionated antiserum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunofluorescence: suitable |
适用性 | not suitable for Western blot not suitable for immunohistochemistry (Paraffin) |
NCBI登记号 | NM_000090.3 |
UniProt登记号 | P02461 |
运输 | wet ice |
Gene Information | human ... COL3A1(1281) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich