产品说明
特异性
Recognizes Human type IV collagen, alpha-2 chains.
免疫原
Human amniotic type IV collagen
应用
Research Category
Cell Structure
Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins
目标描述
170 kDa
外形
Unpurified
Liquid.
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 23IIC3, monoclonal |
species reactivity | monkey, human, rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG1 |
UniProt登记号 | P08572 |
运输 | dry ice |
Gene Information | human ... COL4A2(1284) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich