产品说明
一般描述
Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).
免疫原
KLH-conjugated linear peptide corresponding to human Complex I-75kD.
应用
Detect Complex I-75kD using this rabbit polyclonal antibody, Anti-Complex I-75kD Antibody validated for use in western blotting & IHC (Paraffin).
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Complex I-75kD in 10 µg in human brain and human heart tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Complex I-75kD in human kidney and human cardiac myocytes tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
质量
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Complex I-75kD in 10 µg of mouse brain tissue lysate.
目标描述
~75 kDa observed
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, rat, mouse |
species reactivity (predicted by homology) | orangutan (based on 100% sequence homology), bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI登记号 | NP_004997 |
UniProt登记号 | P28331 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich