产品说明
一般描述
Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
特异性
Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.
免疫原
A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.
Epitope: C-terminus
应用
Research Category
Cell Structure
Western Blot (colorimetric detection):
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.
Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.
Research Sub Category
Adhesion (CAMs)
质量
Evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
目标描述
43-47 kDa
外形
Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.
Format: Purified
Protein A purified
储存及稳定性
Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
分析说明
Control
Mouse brain tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 4E6.2, monoclonal |
species reactivity | pig, mouse, canine, rat |
species reactivity (predicted by homology) | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_000165.3 |
UniProt登记号 | P17302 |
运输 | wet ice |
Gene Information | human ... GJA1(2697) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich