产品说明
一般描述
Cox-1, also known as Cytochrome C oxidase subunit 1, Cytochrome C oxidase polypeptide I, and encoded by the gene MT-CO1, CO1, COX1, MTCO1, is the catalytic subunit of Cytochrome C oxidase that forms the final reduction of oxygen to water in the respiratory chain. Subunits 1-3 form the functional core. Electrons originating in Cytochrome C are transferred via the copper A center of subunit 2 and the heme A of subunit 1 to the bimetallic center formed by the heme A3 and Copper B. The Cox-1 gene is encoded in the mitochondrion and the protein localizes to the inner mitochondrion membrane. It is expressed in all cells that contain functional mitochondria and mutations in Cox-1 are associated with various mitochondrial dysfunctional diseases that manifest themselves in defective vision, anemia, cardiomyopathy, muscle weakness and even some forms of cancer.
免疫原
KLH-conjugated linear peptide corresponding to human Cox-1.
应用
Detect Cox-1 using this Anti-Cox-1 antibody, clone 5C5.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Cox-1 in human thalamus, human kidney and human cerebellum tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cox-1 in 10 µg of HeLa cell lysate.
目标描述
~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 5C5.1, monoclonal |
species reactivity | human |
浓度 | 1 mg/mL |
technique(s) | immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | YP_003024028 |
UniProt登记号 | P00395 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich