Anti-DRD4 Antibody, clone 2B9

产品说明

一般描述

Five subtypes of dopamine receptors exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). As members of the G protein-coupled receptor (GPCR) family, dopamine receptors initiate their biological function by coupling to GTP regulatory proteins (G proteins). D1-like receptors, DRD1 and DRD5, stimulate adenylyl cyclase whereas D2-like receptors, DRD2, DRD3 and DRD4, are coupled to inhibitory G proteins and their stimulation leads to the inhibition of adenylyl cyclase activity, and subsequent respective cellular signaling. All five subtypes have seven transmembrane domains, an extracellular amino terminus, and an intracellular carboxyl terminus. Allelic variants of the D4 dopamine receptor (DRD4) are known characterized risk factors of ADHD. It is reported that hypoxia enhances DRD4 promoter activity by several fold. It is also thought that dopamine receptors such as DRD4 are involved in neuroexcitation and in a variety of other neurological and psychiatric disorders.

免疫原

Linear peptide corresponding to human DRD4.

应用

This Anti-DRD4 Antibody, clone 2B9 is validated for use in WB, IC for the detection of DRD4.
Research Category
Neuroscience
Western Blot Analysis: 0.5 µg/mL from a representative lot detected DRD4 in 10µg of rat brain tissue lysate.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected DRD4 in Ntera-2 cells and in rat brain neurons. (Wolstencroft, E.C., et al. (2007). J Mol Histol. 38(4):333-340.)
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected DRD4 in 10µg of mouse brain tissue lysate.

目标描述

~ 48 kDa observed.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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