产品说明
一般描述
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. (provided by RefSeq)
免疫原
ENDOG (AAH04922.1, 50 a.a. ~ 297 a.a) full-length human protein.
Sequence
ELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK
应用
Anti-ENDOG, (C-terminal) antibody produced in mouse is suitable for western blot analysis.
生化/生理作用
ENDOG (Endonuclease G) is a mitochondrial apoptotic DNase involved in the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. During mitochondrial release and nuclear translocation of EndoG, BNIP3 interacts with the voltage-dependent anion channel (VDAC) to facilitate the process in the nucleus, it cleaves chromatin apoptotic DNA without the need of caspase activity. ENDOG causes DNA fragmentation during and after apoptosis. It is linked with cardiac hypertrophy and Parkinson′s disease.
外形
Solution in phosphate buffered saline, pH 7.4
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~27.39 kDa |
| species reactivity | human |
| technique(s) | western blot: 1 μg/mL |
| NCBI登记号 | BC004922 |
| UniProt登记号 | Q14249 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ENDOG(2021) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
