产品说明
一般描述
Endomucin is an endothelial sialomucin belonging to a class of glycoproteins characterized by their high levels of O-glycosolation and rich quantities of sialic acid. It inhibits focal adhesion assembly and hinders extracellular matrix and cell interaction. Observed expression is highest in kidney heart, brain, liver, lung, spleen, and thymus. Endomucin is only found in endothelial cells.
免疫原
Mouse endothelial cells
应用
Anti-Endomucin Antibody, clone V.5C7 detects level of Endomucin & has been published & validated for use in WB, IP, IH, FC.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP of bEND cell lysate (Morgan, 1999).
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in immunhistochemical staining of frozen and paraffin-embedded tissue sections of mouse tongue, heart, uterus, brain, and small intestine (Morgan, 1999).
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC of transfected COS-7 cells (Morgan, 1999).
Research Sub Category
Organelle & Cell Markers
Adhesion (CAMs)
质量
Evaluated by Western Blot on mouse endothelioma cell line, bEND3, cell lysate.
Western Blot Analysis: 0.0025 µg/mL of this antibody detected Endomucin in 10 µg of bEND3 cell lysate.
目标描述
~ 75kDa observed
外形
Protein G
Format: Purified
分析说明
Control
bEND3 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | rat |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | V.5C7, monoclonal |
species reactivity | mouse |
technique(s) | flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_001156994 |
UniProt登记号 | Q9R0H2 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich