产品说明
特异性
Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
免疫原
Human fascin
应用
Research Category
Cell Structure
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
外形
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Format: Purified
储存及稳定性
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
分析说明
Control
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 55K2, monoclonal |
species reactivity | gerbil, human, sea urchin, echinoderm |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG1 |
适用性 | not suitable for immunoprecipitation |
NCBI登记号 | NM_003088.2 |
UniProt登记号 | Q16658 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich