Anti-Ferrochelatase

产品说明

一般描述

Ferrochelatase, mitochondrial (UniProt: P22830; also known as EC: 4.99.1.1, Heme synthase, Protoheme ferro-lyase) is encoded by the FECH gene (Gene ID: 2235) in human. Ferrochelatase catalyzes the terminal reaction of the heme biosynthetic pathway in all cells. It catalyzes the insertion of ferrous iron into protoporphyrin IX, yielding heme. In eukaryotic cells, it is located in the inner mitochondrial membrane with its active site facing the mitochondrial matrix. Human ferrochelatase is a homodimer with each subunit composed of five regions: a mitochondrial localization sequence, the N terminal domain, two folded domains, and a C terminal extension. The active pocket of ferrochelatase consists of two hydrophobic "lips" and a hydrophilic interior. The hydrophobic lips face the inner mitochondrial membrane and facilitate the passage of the poorly soluble protoporphyrin IX substrate and the heme product via the membrane. Reduced levels of ferrochelatase activity are observed in patients with protoporphyria. Point-mutations in the FECH gene are reported to be associated with certain cases of erythropoietic protoporphyria.

特异性

This polyclonal antibody detects ferrochelatase in human and murine species. It targets the mature form of ferrochelatase.

免疫原

Flag-tagged recombinant fragment corresponding to the mature mitochondrial form containing 369 amino acids (aa 55-423) from human Ferrochelatase.

应用

Anti-Ferrochelatase, Cat. No. ABS2124, is a rabbit polyclonal antibody that detects Ferrochelatase, mitochondrial. It has been tested for use in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells (Karr, S.R., et. al. (1988). Biochem J. 254(3):799-803).

Western Blotting Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells and transfected heterologous cells. (Chen, W., et. al. (2010). Blood. 116(4):628-30; Anand, S., et. al. (2009). Clin Cancer Res. 15(10):3333-43; Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).

质量

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ferrochelatase in 10 µg of K562 cell lysate.

目标描述

~43 kDa observed; 47.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Unpurified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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