Anti-FFAR2 Antibody/GPR43

产品说明

一般描述

Free fatty acid receptor 2 (FFAR2), also known as G-protein coupled receptor 43 (GPR43), functions as the cell membrane receptor for short chain fatty acids that ultimately stimulates G protein coupled, inhibitory (Gi) signals via the adenylate cyclase pathway. FFAR2/GPR43 is found expressed in leukocytes in the lamina propria of the small intestine mostly but some expression can be also be found in splenocytes, adipose tissue, and enteroendocrine cells of the gut. Interesting, FFAR2/GPR43 because it senses short chain fatty acids which are often key metabolites of gut microbiota, it may serve as a biological sensor to regulate the activity of regulatory T cells and the immune response in diseases such as colitis.

免疫原

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human FFAR2/GPR43.
Epitope: Cytoplasmic domain

应用

Research Sub Category
Apoptosis - Additional
This Anti-FFAR2 Antibody/GPR43 is validated for use in Western Blotting and Immunohistochemistry for the detection of FFAR2/GPR43.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected FFAR2/GPR43 in 10 µg of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.

质量

Evaluated by Western Blotting in CHEM-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.

目标描述

~47 kDa observed. The calculated molecular weight of this protein is 37 kDa, but can be observed at ~47 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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